Friederike G. E. Kunz, Dr. rer. nat.

Member of the Research Training Group, 15.08.2011 - 14.08.2013


supervising - past


In vitro analysis of calcium channel function in osteoblasts


This research aspect is part of the project area A: “implant systems for bone regeneration” in cooperation with sub-project A-4.

Chemical, physical and electrical characteristics of biomaterial surfaces determine cellular acceptance and integration into the host tissue. Adequate cell adhesion is essential for subsequent cell function and occupation of artificial surfaces. Here, the focus is on the regulation of voltage gated calcium channels by prominent cellular adhesion components like the actin cytoskeleton. Complementary to the current studies within A-4, actin reorganization due to microstructured titanium surface is analyzed with regard to consequences for calcium entry. To approximate experimental conditions to aged or compromised bone, an artificial in vitro model was established and included. Experiments are performed using the human osteoblastic cell line MG-63 cultured on either chemically modified or geometrically microstructured titanium. Methods comprise immunocytochemistry, CLSM, flow cytometry, patch clamp and calcium imaging techniques.


Peer-reviewed Journal Papers:

KUNZ, F., REBL, H., QUADE, A., MATSCHEGEWSKI, C., FINKE, B. and NEBE, J.B., 2015. Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerized allylamine. European Cells and Materials. 29, pp. 177-189.   IF 4,887
BERGEMANN, C., KUNZ, F., QUADE, A. and NEBE, J.B., 2014. Zellmigration in vitro in einem artifiziellen 3D Layer-Modell. In: Knöcherne Geweberegeneration: Zellphysiologie im Dreidimensionalen. Hans-Georg Neumann, Ernst-Dieter Klinkenberg (Herausgeber), Shaker Verlag GmbH, Aachen 2014, ISBN 987-3-8440-3049-5. pp 131-142.
STAEHLKE, S., KUNZ, F., LOEFFLER, R., MATSCHEGEWSKI, C., FLEISCHER, M., KERN, D. P. and NEBE J. B., 2013. Regulation of T-type calcium channels in osteoblasts on micro-structured surface topography. Advanced Materials Research, 647, pp.  476-481.
NEBE, J.B., CORNELSEN, M., QUADE, A., WEISSMANN, V., KUNZ, F., OFE, S., SCHRÖDER, K., FINKE, B., SEITZ, H. and BERGEMANN, C., 2012. Osteoblast Behavior in vitro in Porous Calcium Phosphate Composite Scaffolds, Surface Activated with a Cell Adhesive Plasma Polymer Layer. Materials Science Forum. Trans Tech Publications, Switzerland 706-709, pp .566-571.
BERGEMANN, C., QUADE, A., KUNZ, F., OFE, S., KLINKENBERG, E.-D., LAUE, M., SCHROEDER, K., WEISSMANN, V., HANSMANN, H., WELTMANN, K.-D. and NEBE, J.B., 2011. Ammonia Plasma Functionalized Polycarbonate Surfaces Improve Cell Migration Inside an Artificial 3D Cell Culture Module. Plasma Processes and Polymers. 9, pp. 261-272.
WYRWA, R., MUELLER, U., BERGEMANN, C., KUNZ, F., NEBE, B. and SCHNABELRAUCH, M., 2011. Der Effekt endogener Amine in elektrogesponnenen Polylactid-Scaffolds auf die Zelladhäsion. 7. Thüringer Grenz- und Oberflächentage und 8. Thüringer Biomaterial-Kolloquium, Tagungsband, ISBN 978-3-00-035347-5, pp. 319-323.
KUNZ, F., BERGEMANN, C., KLINGENBERG, E-D., WEIDMANN, A., LANGE, R., BECK, U. and NEBE, J.B., 2010.  A novel modular device for 3-D bone cell culture and non-destructive cell analysis. Acta Biomater. 6, pp. 3798-3807.

Peer-reviewed Conference Papers:

STÄHLKE, S., KUNZ, F., MATSCHEGEWSKI, C., NEBE, J.B., 2013. Analyse der intrazellulären Kalzium Mobilisierung in Osteoblasten auf definierter Mikrostruktur. Deutsche Gesellschaft für Biomaterialien (DGBM) ISSN 2193-0651 BIONANO-MATERIALS, 14, p.81, Erlangen --> DGBM Posterpreis.
STAEHLKE, S., KUNZ, F., LOEFFLER, R., MATSCHEGEWSKI, C., FLEISCHER, M., KERN, D.P., NEBE, J.B., 2013. Intracellular signaling in osteoblasts on regularly structured titanium surfaces. 6th Annual meeting Scandinavian Society for Biomaterials (ScSB) Materials for Tissue Engineerings, Hafjell, Norway.
STAEHLKE, S., KUNZ, F. and NEBE, J.B., 2013. Intracellular calcium mobilization in osteoblasts in dependance on defined surface micro structures. BioInterface, Minneapolis, USA.
KUNZ, F., QUADE, A., MATSCHEGEWSKI, C., STAEHLKE, S., BIRKHOLZ, H., REBL, H., STEFFEN, J. and NEBE, J.B., 2012. Actin cytoskeleton mediated cell response of osteoblasts to plasma amino functionalised titanium surfaces. 4th International Symposium Interface Biology of Implants (IBI) 2012, Rostock, Germany, Abstracts, ISBN 978-3-00-038220-8,pp.49.
STEFFEN, J., STAEHLKE, S., MATSCHEGEWSKI, C., KUNZ, F., LOEFFLER, R. and NEBE, JB., 2012.  Correlation of calcium channel expression and actin organization – bone cells on microstructures. BioNanoMaterials, 13 (1-4), pp. 219.