MSc Sarah Zaatreh
University Medicine Rostock
Department of Orthopaedics
Microbiological investigations of antibacterial implant surfaces in mono- and co-culture
Total joint arthroplasty is one of the most frequent and effective surgeries today. However, despite improved surgical techniques, a significant number of implant-associated infections still occur. Suitable in vitro models are needed to test potential approaches to prevent infection. We aimed to establish an in vitro co-culture setup of human primary osteoblasts and S. epidermidis to model the onset of implant-associated infections, and to analyze antimicrobial implant surfaces and coatings.
- Co-cultures were performed as a single-species infection on the osteoblasts with S. epidermidis incubated for 2 and 7 days under aerobic conditions
- Planktonic S. epidermidis was quantified by centrifugation and determination of colony-forming units
- Quantification of biofilm-bound S. epidermidis on the test samples was performed by sonication and colony-forming unit counting
- Vital primary osteoblasts on the test samples was performed by trypan-blue staining and counting
- Scanning electron microscopy was used for evaluation of topography and composition of the species on the sample surfaces
Peer-reviewed journal papers:
- ZAATREH, S., HAFFNER, D., STRAUSS, M., DAUBEN, T., ZAMPIONI,C., MITTELMEIER, W., QUANDT, E., KREIKEMEYER ,B. and BADER, R., 2017. Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model. Molecular Medicine Reports. DOI: 10.3892/mmr.2017.6218.
- WARNKE, P., REDANZ, S., ZAATREH, S., PODBIELSKI, A., 2016. Augmented recovery of microorganisms from swabs by homogenization: a novel standardizable high-throughput approach. Diagn Microbiol Infect Dis. Jan;84(1):16–8.
- ZAATREH, S., WEGNER., K, STRAUSS, M., PASOLD, J., MITTELMEIER, W., PODBIELSKI, A., KREIKEMEYER ,B., BADER,R., 2016. Co-Culture of S. epidermidis and Human Osteoblasts on Implant Surfaces: An Advanced In Vitro Model for Implant-Associated Infections. PloS One.11(3):e0151534.
- DAUBEN, T.J., ZIEBART ,J., BENDER, .T, ZAATREH, S, A., KREIKEMEYER ,B., BADER,R., 2016. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Res Int. 2016:5178640.
- ZAATREH, S., ENZ, A., KLINDER; A., KÖNIG, T., MITTELMEIER, L., KUNDT, G., MITTELMEIER, W., 2016. Prospective data collection and analysis of perforations and tears of latex surgical gloves during primary endoprosthetic surgeries. GMS Hyg Infect Control. 2016;11:Doc25. DOI: 10.3205/dgkh000285, URN: urn:nbn:de:0183-dgkh0002852.
Peer-reviewed paper important congresses:
- ZAATREH, S., HAFFNER, D.,PASOLD, J., KREIKEMEYER ,B., MITTELMEIER, W., PODBIELSKI, A., BADER,R. 2015. In-vitro analysis of the antimicrobial properties of magnesium-coated titanium implantsTrack A. Biomaterials and Biocompatibility 1. Biomed Tech (Berl)..60 Suppl 1:S1–30.