Dipl.-Biochem. Philipp Wysotzki
Connection between surface structure, cell activity and cell adhesion power
The aim of this project is to compare the initial adhesion of prokaryotic and eukaryotic cells on different surfaces. A profound approach to observe the initial adhesion process is single cell force microscopy (SCFM), an atomic force microscopy based technique. In the ongoing work we establish a method, which enables to analyze the adhesion with a single cell on multiple surfaces. These method is used to screen for a surface materials or coating which suppresses the adhesion of prokaryotic relative to eukaryotic cells in order to reduce the inflamation risk after implant surgery.
- Test of different cantilever coatings
- Develop and improve SCFM on multiple surfaces
- Test poly-dopamine based surfaces coatings
- Comparison of initial adhesion strength of prokaryotes and eukaryotes
- A protocol to affix eukaryotic (MC3T3), primary neuronal mouse as well as prokaryotic cells (Staph. capitis sub. capitis) to a cantilever for single cell force measurements (SCFMs) has been established.
- SCFMs with the mentioned cell types on different cell adhesion promoting surface coatings were successfully performed.
- A positive influence of an alkaline pH on the initial adhesion of osteoblast on pure and collagen coated titanium was demonstrated with SCFM.
Peer-reviewed Journal Papers:
GALOW, A., WYSOTZKI, P., BAUMANN, W. and GIMSA, J., 2016. Enhancing the regeneration of bone defects by alkalizing the peri-implant zone – an in vitro approach. Current Directions in Biomedical Engineering. 2(1), pp. 547–551. DOI: 10.1515/cdbme-2016-0121.
Peer-reviewed Conference Papers:
WYSOTZKI, BAUMANN, W. ,GIMSA, J. 2016. Comparison of the adhesion of single prokaryotic and eukaryotic cells to different surfaces using single cell force microscopy. DECHEMA Single Cell Technologies, Frankfurt.
WYSOTZKI, P., GALOW, A. ,BREHME, H.,GIMSA, J. 2015. Single cell force microscopy method to investigate the adhesion of a single cell on multiple surfaces. 5th International Symposium Interface Biology of Implants, Rostock.