Dr. rer. nat. Claudia Matschegewski

Member of the Research Training Group, 1.10.2008 - 31.12.2012

PhD Defence 31.05.2013, Summa Cum Laude

Analyse initialer Adhäsionsmechanismen von humanen Osteoblasten in Abhängigkeit physiko-chemischer Oberflächencharakteristika definiert mikrostrukturierter Titanoberflächen

Topic

Analysis of time dependent adhesion mechanisms of osteoblasts in dependence on chemical, physical and electrical characteristics of surfaces with geometrical microstructures

Description

Knowledge about the complexity of cellular behavior in dependence on physico-chemical characteristics of a biomaterial is of clinical relevance for the development and improvement of implant designs. This study aims at the investigation of the cellular physiology of human MG-63 osteoblasts on micro-scaled titanium arrays with regular surface geometry. Here we want to find out correlations of cell-biological parameters with physico-chemical material characteristics to predict and further direct cellular behavior.

Approach

  1. Application of a range of micro-scaled surface structures with regular geometry as an artificial model for the systematic study of cellular behavior in response to surface topography.
  2. Extension of existing work on stochastic material surfaces.
  3. Interdisciplinary approaches to improve the data correlation in investigations on the cell-biomaterial dialogue.

Results

Revelation of direct surface topographical effects on cell behavior:

  1. Adaptation of cell morphology and intracellular structures (actin cytoskeleton) to the underlying surface geometry.
  2. Functional dependency between cell architecture and cell physiology.
  3. Direct quantitative relationships between cell behavior and physico-chemical surface characteristics.

Publications

Peer-reviewed Journal Papers:

KUNZ, F., REBL, H., QUADE, A., MATSCHEGEWSKI, C., FINKE, B. and NEBE, J.B., 2015. Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerized allylamine. European Cells and Materials. 29, pp. 177-189.   IF 4,887

STAEHLKE, S., KUNZ, F., LOEFFLER, R., MATSCHEGEWSKI, C., Fleischer, M., KERN, D. P. and NEBE J. B., 2013. Regulation of T-type calcium channels in osteoblasts on micro-structured surface topography. Advanced Materials Research, 647, pp.  476-481, DOI: 10.4028/www.scientific.net/AMR.647.476

MATSCHEGEWSKI, C., STÄHLKE, S., BIRKHOLZ, H., LÖFFLER, R., KERN, D., ENGEL, K., NEBE, B., 2012. Quantitative analysis of the cellular actin cytoskeleton on geometrically designed surface topography. Materials Science Forum, 706-709, pp. 543-548.

LOEFFLER, R., FLEISCHER, M., MATSCHEGEWSKI, C., STAEHLKE, S., LANGE, R., NEBE, J.B., KERN, D.P., 2012. Pyramid Array Substrates for Biomedical Studies. Journal of Vacuum Science and Technology.  B 30. DOI: 10.1116/1.4757109.

MATSCHEGEWSKI, C., STAEHLKE, S., BIRKHOLZ, H., LANGE, R., BECK, U., ENGEL, K. and NEBE, JB.,2012. Automatic Actin Filament Quantification of Osteoblasts and their Morphometric Analysis on Microtextured Silicon-titanium Arrays. Materials, 5, pp. 1176-1195. DOI:10.3390/ma50711762012.

KABASO, D., GONGADZE, E., PERUTKOVÁ, Š., MATSCHEGEWSKI, C., KRALJ-IGLIC, V., BECK, U., VAN RIENEN, U. and IGLIC, A., 2011. Mechanics and electrostatics of the interactions between osteoblasts and titanium surface. Computer Methods in Biomechanics and Biomedical Engineering, 14(5), pp 469-482.

MATSCHEGEWSKI, C., STAEHLKE, S., LÖFFLER, R., LANGE, R., CHAI, F., KERN, D., BECK, U. and NEBE, J.B., 2010. Cell architecture-cell function dependencies on titanium arrays with regular geometry. Biomaterials, 31(22), pp. 5729-5740. doi:10.1016/j.biomaterials.2010.03.073.

LANGE, R., ELTER, P., BIALA, K., MATSCHEGEWSKI, C., STÄHLKE, S., LÖFFLER, R., FLEISCHER, M., NEBE, J.B., KERN, D. and BECK, U., 2010. Titanium surfaces structured with regular geometry-material investigations and cell morphology. Surface and Interface Analysis, 42(6), pp. 497-501.

Reviewed Paper Important Congresses:

STAEHLKE, S., KUNZ, F., LOEFFLER, R., MATSCHEGEWSKI, C., FLEISCHER, M., KERN, D. P. and NEBE J. B., 2012. Regulation of T-type Calcium Channels in Osteoblasts on Micro-Structured Surface Topography. 2012 International Conference on Biomaterial and Bioengineering (ICBB), Hong Kong, China. pp. 21.

KUNZ, F., QUADE, A., MATSCHEGEWSKI, C., STAEHLKE, S., BIRKHOLZ, H., REBL, H., STEFFEN, J. and NEBE, J.B., 2012. Actin cytoskeleton mediated cell response of osteoblasts to plasma amino functionalised titanium surfaces. 4th International Symposium Interface Biology of Implants (IBI) 2012, Rostock, Germany, Abstracts, ISBN 978-3-00-038220-8, pp.49.

STEFFEN, J., STAEHLKE, S., MATSCHEGEWSKI, C., KUNZ, F., LOEFFLER, R. and NEBE, JB., 2012.  Correlation of calcium channel expression and actin organization – bone cells on microstructures. BioNanoMaterials, 13 (1-4), pp. 219.

KUNZ, F., QUADE, A., SCHROEDER, K., MATSCHEGEWSKI, C. and NEBE, B., 2011.  Impact of plasma polymer deposition on titanium on osteoblasts with disturbed cell functions due to impaired actin cytoskeleton. BIOmaterialien 12(1-4), pp. 199

MATSCHEGEWSKI, C., BIRKHOLZ,  H., STAEHLKE, S., LANGE, R., BECK, U., ENGEL, K. and NEBE, J.B., 2011. Determination of cellular phenotype and actin quantification of osteoblasts on geometrically defined microtextured titanium arrays. BIOmaterialien 12(1-4), pp. 113.

MATSCHEGEWSKI, C., BIRKHOLZ,  H., STAEHLKE, S., LOEFFLER, R., KERN, D., ENGEL, K. and NEBE, J.B. 2011. Quantification of intracellular structures as a tool for the correlation of material vs. cellular parameters. BMT 2011, Freiburg

STAEHLKE, S., MATSCHEGEWSKI, C., LOEFFLER, R.,  LANGE, R., BECK, U., KERN, D. and NEBE, J.B. 2011.  Analyse der Interaktion von Osteoblasten auf mikrostrukturierten Oberflächen in Bezug auf die Organisation des Aktinzytoskeletts. BIOmaterialien 12(1-4), pp. 115.

MATSCHEGEWSKI, C., BIRKHOLZ,  H., STAEHLKE, S., LANGE, R., BECK, U.,  ENGEL, K. and NEBE, J.B. 2011. Cell morphometric analysis of osteoblasts on geometrically microstructured titanium surfaces – basic research for orthopedic implants. World Congress on regenerative medicine (WCRM) 2011 Leipzig.

MATSCHEGEWSKI, C., STAEHLKE, S., LOEFFLER, R., LANGE, R., CHAI, F., KERN, D.P., BECK, U. and NEBE, J.B., 2010. Cell Architecture-Cell Function Dependencies on Micro-Arrays with Cubic Pillar Structures. Deutsche Gesellschaft für Materialkunde, DGM, Material Science and Engineering, Topic F, Biomat- F3, Interfaces, Darmstadt.

NEBE, J.B., REBL, H., FINKE, B., MATSCHEGEWSKI, C., STÄHLKE, S., BECK, U., KERN, D.P., WELTMANN, K.D. and SCHRÖDER, K., 2010. Plasmachemisch modifizierte Metalloberflächen und ihr Einfluss auf die Zellphysiologie. Workshop on Metallic Biomaterials, Geesthacht, abstract Booklet, p 7.

REBL, H., FINKE, B., MATSCHEGEWSKI, C., STAEHLKE, S., LANGE, R., KERN, D.P., BECK, U., SCHROEDER, K. and NEBE, J.B., 2010. Cell architecture on plasmafunctionalized, structured metal surfaces. 23 th ESB 2010, CD abstract no. H 3492, Tampere, Finnland.

LANGE, R., ELTER, P., MATSCHEGEWSKI, C., WEIDMANN, A., LÖFFLER, R., FLEISCHER, M., NEBE, J.B., KERN, D. and BECK, U., 2009. Material properties of Titanium surfaces with regular geometry and their influence on osteoblast behaviour. ESB, Lausanne.

LANGE, R., ELTER, P., BIALA, K., MATSCHEGEWSKI, C., STÄHLKE, S., LÖFFLER, R., FLEISCHER, M., NEBE, J.B., KERN, D. and BECK, U., 2009. Titanium Surfaces Structured with Regular Geometry - Material and Cell Biological Investigations. ECASIA, Antalya, Surf. Interface Anal. 42, pp. 497–501; DOI 10.1002/sia.3320

BIRKHOLZ, H., MATSCHEGEWSKI, C., NEBE, J.B. and ENGEL, K., 2009. Quantification of actin filament organization by estimating graph structures in confocal microscopic images. ISBN 978-3-642-03881-5, IFMBE Proceedings, 25 (4), pp 1932-1935.

NEBE, J.B., JESSWEIN, H., WEIDMANN, A., FINKE, B., MATSCHEGEWSKI, C., LANGE, R., BECK, U. and SCHROEDER, K., 2009. Sensitivity of Osteoblasts to Topographical and Chemical Features of Metal Implants. ICMAT, Singapore.

Supervisors